cd45 2147 sm fluidigm 3147004b  (fluidigm)

Journal: bioRxiv

Article Title: Bigh3 is essential for pulmonary fibrosis

doi: 10.1101/2025.09.02.673852

Figure Lengend Snippet: a , schematic of Bigh3 −/− transgenic mouse, b, abundance of Bigh3 in the lungs of Bigh3 +/+ and Bigh3 −/− mice, c, representative images of Bigh3 +/+ and Bigh3 −/− and hematoxylin and eosin (top) and Masson’s Trichome stained (bottom) stained lungs, d, whole body weight, e, number of cells and f, number of cells per milligram of tissue in the lungs of Bigh3 +/+ and Bigh3 −/− mice, g, graphical schematic of retro-orbital CD45.2-147Sm antibody injection, h, representative gating of CD45-, CD45+, CD45.2-, and CD45.2+ cells, i, UMAPs of CD45+CD45.2-(top) leukocytes and CD45-stromal cells in the lungs Bigh3 +/+ and Bigh3 −/− mice j, enumeration of leukocyte and stromal cells in the lungs of Bigh3 +/+ and Bigh3 −/− mice, data shown as mean +SEM. All comparisons between Bigh3 −/− and Bigh3 +/+ mice were determined by a student’s t-test (d-f) or multiple unpaired t test (j; only endothelial cells displayed a significant difference between groups). Each symbol represents 1 mouse in b, d-f. Data are from 1 (b) or 2 (d-j) experiments with greater >= 7 mice per group (d-j).

Article Snippet: Animals were injected retro-orbitally using an insulin syringe (BD; NJ, USA; Cat. 305932) with 3ug CD45.2 147Sm (Standard Biotools; ON, CAN; Cat. 3147003B) in 100-125uL of sterile saline.

Techniques: Transgenic Assay, Staining, Injection

Journal: STAR Protocols

Article Title: Functional phenotyping of hepatic lymphocytes in murine MASH by mass cytometry

doi: 10.1016/j.xpro.2023.102743

Figure Lengend Snippet:

Article Snippet: Mouse anti-mouse CD45.2-147Sm (clone: 104) dilution (1:100) , Standard BioTools , Cat# 3147004B.

Techniques: Recombinant, Red Blood Cell Lysis, Staining, Antibody Labeling, Software